DNA Extraction and Purification
Biochain Institute Inc Genomic DNA - Multiple Sclerosis Disease: Brain: Temporal Lobe, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Biochain Institute Inc Genomic DNA - Human Diabetic Diseased Tissue: Small Intestine: Jejunum, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
Bioworld Endotoxin Removal Kit (Mini) - For DNA, 20 Prep(s)
For the rapid removal of endotoxins from previously purified DNA Reduce endotoxin levels to 0.1 EU/µg DNA or less Effectively remove endotoxins in as little as 20 minutes Remove endotoxins from up to 40 µg of DNA Rapid spin-column formatFEATURES AND BENEFITSFast and easy processing - Rapid spin-column format allows for the processing of multiple samples in 20 minutes Endotoxin-free plasmid DNA- Using Norgen’s Endotoxin Removal Kit (Mini), endotoxin levels are reduced to less than or equal to 0.1 EU/µg plasmid DNA High recovery of input DNA - Average DNA recovery is > 90% of the input amount Recovered DNA is suitable for downstream applications - Purified DNA is fully compatible with sequencing, cloning and transfections
Zymo Research Corporation ZR BashingBead™ Lysis/Filtration Tubes w/ 0.5 mm Beads (50 ml) (25 Pack)
The state of the art, ultra-high density beads are fracture resistant, chemically inert, and ideal for disrupting tissues (2.0 mm) or microbes (0.5 mm). Used in the Quick-DNA Fungal/Bacterial Midiprep kit (Cat. #D6105).
Zymo Research Corporation EZ Nucleosomal DNA Prep Kit, 20 preps
Atlantis dsDNase is a double-strand DNA specific endonuclease that cleaves phosphodiester bonds in DNA to yield oligonucleotides with 5’- phosphate and 3’-hydroxyl termini. Atlantis dsDNase digestion yields very homogeneous populations of core nucleosomes and purification of the nucleosome-associated DNA is performed using Zymo Research’s proven Fast-Spin column technology. The result is pure nucleosomal DNA ready for analysis in less than 45 minutes.
![Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1](https://assets.fishersci.com/TFS-Assets/CCG/product-images/VN00062073-D4302-ZYMOBIOMICS-96-MAGBEAD-DNA-K.JPG-250.jpg)
Zymo Research Corporation ZymoBIOMICS DNA 96 MagBead Kit (2 x 96 preps)Includes: [D4306] ZymoBIOMICS 96 MagBead Kit x 1, [S6002-96-4] ZR BashingBead Lysis Racks (0.1mm & 0.5 mm) 2 pack x 1[D4300-1-150] ZymoBIOMICS Lysis Solution, 150 ml x 1
The ZymoBIOMICS DNA Kits are designed for purifying DNA from a variety of sample inputs that is immediately ready for microbiome or metagenome analyses. The ZymoBIOMICS lysis system eliminates bias associated with unequal lysis efficiencies of different organisms (e.g. Gram-negative/positive bacteria, fungus, protozoans, and algae), making it ideal for microbial community profiling. Uniform mechanical lysis of all microbes is achieved by bead beating with the innovative ultra-high density BashingBeads. This kit is equipped with our OneStep PCR Inhibitor removal technology, enabling PCR amplification from DNA derived from inhibitor-rich environmental samples. Purified DNA is ideal for all downstream applications including PCR, arrays, 16S rRNA gene sequencing, and shotgun sequencing. DNA Size is 15-20 kb.
New England Biolabs, Inc. NEBNext Library Quant Kit for Illumina – 500 reactions
The NEBNext Library Quant Kit components have been optimized to deliver significant improvements to qPCR-based library quantitation for Illumina sequencing. The kit contains primers which target the P5 and P7 Illumina adaptor sequences, and a set of four high-quality, pre-diluted DNA standards to enable reliable quantitation of diluted DNA libraries between 150–1000 bp.
Zymo Research Corporation Quick-DNA™ Viral 96 Kit (4 x 96 Preps)
Quick recovery of viral DNA from a wide range of sources using Zymo-Spin plate technology. Plate design allows DNA to be eluted at high concentrations into minimal volumes. Eluted DNA is suitable for PCR, Southern blotting, and restriction endonuclease digestion.
Biochain Institute Inc Genomic DNA - Liver Cirrhosis: Pancreas, from a single donor, 50 ug/PK
BioChain's tissue genomic DNAs are isolated from a wide variety of tissues using a proprietary modified guanidine thiocyanate technique. The quality and purity of genomic DNA are tested by spectrophotometer and electrophoresis. A260/280 is between 1.8 and 2.0, and A260/230 is >2.0 (detected in 10 mM Tris-Cl, pH 7.5). Contamination of RNA, polysaccharides and proteoglycans has been effectively eliminated.
LGC GENOMICS LLC (Lucigen) 1000mL; Simple, rapid extraction of PCR-ready DNA for screening and genotyping type applications from hair follicles, quill-end cells of feathers, tissue-culture cells, buccal cells and other samples
QuickExtract DNA Extraction Solution - 1000mLSimple, rapid extraction of PCR-ready DNA for screening and genotyping type applicationsFast: 8 minute extraction protocol for most sample typesSimple: No centrifugation steps of spin columns used to help increase yieldsAutomation-friendly: Simple protocol integrated easily into automated workflowsSafe: Uses only non-toxic reagentsRecommended for rapid, easy sample prep for T7E1 CRISPR mutation detection assays
New England Biolabs, Inc. WarmStart LAMP Kit (DNA & RNA) – 500 reactions
The WarmStart LAMP Kit (DNA & RNA) is designed to provide a simple, one-step solution for Loop-Mediated Isothermal Amplification (LAMP) of DNA or RNA (RT-LAMP) targets. LAMP and RT-LAMP are commonly used isothermal amplification techniques that provides rapid detection of a target nucleic acid using LAMP-specific primers (supplied by the user) and a strand-displacing DNA polymerase. This kit is supplied with the WarmStart LAMP 2X Master Mix, which contains a blend of Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase in an optimized LAMP buffer solution. Both Bst 2.0 WarmStart DNA Polymerase and WarmStart RTx Reverse Transcriptase have been engineered for improved performance in LAMP and RT-LAMP reactions. A fluorescent dye is also supplied to enable real-time fluorescence measurement of LAMP. The WarmStart LAMP Kit is compatible with multiple detection methods, including turbidity detection, real-time fluorescence detection, and end-point visualization.
Research Products International Corp Zyppy Plasmid Miniprep Kit, 400 Preps
The Zyppy Plasmid Miniprep Kit is a plasmid DNA kit that features a pellet-free modified alkaline lysis system that bypasses the normal cell-pelleting and resuspension steps common to classical plasmid preparation procedures. Simply add the uniquely formulated 7X Lysis Buffer directly to your bacterial culture, neutralize, and then purify with Zymo-Spin column technology. Additionally, the patented colored buffers included in the kit allow for easy visualization of complete cell lysis and neutralization. The Zyppy Plasmid Miniprep Kit is the fastest and easiest method available to separate plasmid DNA from E. Coli efficiently. The plasmid DNA is high quality, endotoxin-free, and well suited for use in transfection, bacterial transformation, restriction endonuclease digestion, DNA ligation, PCR, transcription, sequencing, and other sensitive downstream applications.
Fastest: 8 minutes from culture flask to high-quality plasmid DNA.
Macherey-Nagel NucleoBond Xtra Midi, 10 preps
NucleoBond Xtra Midi (10) 10 preps for the isolation of plasmid DNA - NucleoBond Xtra Midi Columns, NucleoBond Xtra Midi Column Filters, buffers, RNase A
Biochain Institute Inc SeqPure PCR Purification Kit (500 ml), 500 ml/pk
SeqPure PCR reagent is a post PCR and Next Gen library prep clean up system based on paramagnetic beads technology, designed for an efficient purification of PCR amplicons. The purification consists of removal of salts, primers, primer-dimers, dNTPs, as DNA fragments are selectively bound to the magnetic beads particles; and highly purified DNA is eluted with low salt elution buffer or water and can be used directly for downstream applications. The protocol can be adapted to your current liquid handling workstation (e.g. Beckman, Hamilton, Tecan, Caliper, Perkin Elmer, Agilent and Eppendorf) utilizing your current protocol as well as it can be performed manually.